Fig. 6

BBB permeability measurements in P21 mice following 0, 1, 3 or 5 60s injuries. (A) Representative example of whole brain slices stained with (Ai) Ib4 (cyan) to label blood vessels, (Aii) fluorescent dye, TMR (magenta), (Aiii) both Ib4 and TMR, and (Aiv) close up of Ib4 and TMR to show TMR leakage from the vessels. Images were taken at 20x magnification. (B) Quantification of TMR leakage from vessels by subtracting the fluorescence of Ib4 from the fluorescence of TMR. (C) Quantification of AQP4 fluorescence in the (Ci) mPFC, (Cii) lPFC, and (D) HPC of female and male mice. (E) Representative example of AQP4 (green) staining and Hoechst (blue) in the HPC. Images were taken at 20x magnification and scale bars indicate 1000 \(\:\mu\:\)m. Error bars are ± SEM; females are denoted with open circles, and males are denoted with closed circles. Significance at p < 0.05 is denoted with *, and p < 0.01 with **