Fig. 2

15s injury results. (A) Weight gain from P8 to euthanasia at P21. (B) Brain weight of female and male mice at euthanasia on P21. (C) Time for mice to turn upright on negative geotaxis on P9, P11 and P13. (D) Total distance travelled during the open field task and (E) the percent of time they spent within the centre zone of the open field. (F) Heat map of relative gene expression measured with PCR in the (Fi) HPC and (Fii) PFC after a 15s injury. Genes are in alphabetical order and were normalised to 3 housekeeping genes. Asterisks indicate a significant main effect of injury on gene expression. (G) Representative examples of the anterior, medial, and posterior HPC stained with GFAP (red) and Hoechst (blue) taken from a mouse with a single injury. Images were taken at 20x magnification. (H) Percent coverage of positive GFAP signal in the whole HPC. Error bars are \(\:\pm\:\)SEM; females are denoted with open circles, and males are denoted with closed circles. Significance at p < 0.05 is denoted with *, and p < 0.01 with **