Fig. 7
IL-6 induces astrocyte reactivity in a Stat3-dependent manner. Astrocytes were isolated from non-infected, adult Cre+/− mice, treated with IL-6, IL-6 and TAM or mock solution for 72 h and analyzed. (A) Representative Western blots and densitometric analysis of Stat3, p-Stat3, GFAP, and C3 expression normalized per expression of β-actin. (B) Analysis of expression of genes associated with astrocyte reactivity. Expression levels were normalized relative to the expression of the respective gene in mock-treated astrocytes. Data represent mean ± SE, ***p < 0.001, **p < 0.01, *p < 0.05, by one-way ANOVA with Bonferroni multiple comparisons post-hoc test, N = 3 independent astrocyte cultures per group, each originating from an individual animal