Fig. 5

Proteomic profiling reveals radiation-induced leukotriene biosynthesis in the mPFC is reversed by microglial repopulation. (A) Schematic of the microglial repopulation, mPFC tissue collection, microglia cell sorting and mass spectrometry-based proteomics analysis (for male, Sham: n = 2; R + CON: n = 4; R + PLX: n = 3). (B) Gating strategy and representative plots of viable microglia (GFP-positive and DAPI-negative) in the mPFC. (C-D) Volcano plots of the differentially expressed proteins (DEPs) comparing the “R + CON” with “Sham” groups (C) and the “R + PLX” with “R + CON” groups (D). Blue and red dots represent significantly down-regulated and up-regulated DEPs, respectively. (E-F) Plots showed top enriched pathways of DEPs comparing the “R + CON” with “Sham” groups (E), and the “R + PLX” with “R + CON” groups (F), revealed by gene ontology analysis. (G) Venn plot of the overlap DEPs between groups. Bold-labeled numbers represented DEPs with opposite directions of changes in different groups. (H) Heatmap showing the DEPs with opposite directions of changes among three groups in the microglia sorting from the mPFC. Scale bar on the left. Sham: non-irradiated mice without microglial repopulation, R + CON: mice received 15 Gy irradiation without microglial repopulation, R + PLX: mice received 15 Gy irradiation with microglial repopulation