Fig. 8
Clusterin miR-22-5p increases the ability of LRRK2 G2019S-KI primary astrocytes to take up α-syn pffs. (a) Representative fluorescence microscopy images of LRRK2 G2019S-KI astrocytes treated with mouse pHrodo labeled α-syn pffs for 16 h. Scale bar 10 μm. (b) Cumulative frequency distributions of intracellular pHrodo labeled α-syn pffs in LRRK2 G2019S-KI transfected with NC or miR-22-5p mimic (LRRK2 G2019S-KI NC = 167 cells and LRRK2 G2019S-KI miR-22-5p mimic = 175 cells; Kolmogorov-Smirnov test, p < 0.0001). Quantification of intracellular α-syn pffs is calculated as fluorescence intensity/µm2 from three independent experiments (~ 50 cells per experiment)