Fig. 6

Selection and validation of predicted Clu targeting miRNAs. (a) Bioinformatics prediction analysis of miRNAs that target Clu mRNA. (b) miRNAs identified by at least three databases were selected for further analysis. (c) Relative expression of selected miRNAs in LRRK2 KO and WT primary astrocytes normalized on the geometric mean of the reference RNAs and expressed as rq. Data are representative of three independent experiments and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test, miR-22-5p with p = 0.0145 and miR-497-5p with p = 0.0047. (d) Relative expression of selected miRNAs in LRRK2 WT and G2019S-KI primary astrocytes normalized on the geometric mean of the reference RNAs and expressed as rq. Data are representative of three independent experiments and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test, miR-15b-5p with p = 0.0348, miR-16-5p with p = 0.0045 and miR-22-5p with p = 0.0039. While data of miR-195-5p were analyzed using Mann Whitney test. (e) Relative expression of selected miRNAs in the striatum of LRRK2 WT and KO mice normalized on the geometric mean of the reference RNAs and expressed as rq. Data are representative of at least 3 animals (LRRK2 WT = 4 and KO = 3) and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test, miR-15b-5p with p = 0.0376 and miR-22-5p with p = 0.0289. While data of miR-16-5p were analyzed using Mann Whitney test