Fig. 5

LRRK2 controls protein translation in astrocytes without affecting the translation factor 4E-BP. (a) Cell lysates of LRRK2 KO and WT primary astrocytes previously treated with 1µM puromycin for 30 min were subjected to immunoblotting using LRRK2, puromycin and GAPDH antibodies. Data are representative of five independent experiments performed on two different primary cultures and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test; p = 0.0420. (b) Cell lysates of LRRK2 WT and G2019S-KI primary astrocytes previously treated with 1µM puromycin for 30 min were subjected to immunoblotting using puromycin, LRRK2 and GAPDH antibodies. Data are representative of six independent experiments performed on two different primary cultures and are expressed as the mean ± SEM. Data were analyzed using Mann Whitney test; p = 0.0260. (c) Cell lysates of LRRK2 G2019S KI and WT primary astrocytes were subjected to immunoblotting using P-4E-BP and total 4E-BP antibodies. Data are representative of three independent experiments and are expressed as the mean ± SEM