Fig. 4

LRRK2 does not affect Clu gene expression. (a) Quantification of Clu gene expression in LRRK2 G2019S-KI and WT primary astrocytes. The relative expression of Clu was calculated using the comparative Ct (ΔΔCt) method, normalized on the geometric mean of the two HK genes, and expressed as fold change (rq). Data are representative of seven independent experiments performed on two different primary cultures and are expressed as the mean ± SEM. (b) Quantification of Clu gene expression in LRRK2 KO and WT primary astrocytes. The relative expression of Clu was calculated using the comparative Ct (ΔΔCt) method, normalized on the geometric mean of the two HK genes, and expressed as rq. Data are representative of seven independent experiments performed on two different primary cultures and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test, p = 0.0317