Fig. 3

LRRK2 KO mouse brains and primary astrocytes exhibited reduced levels of Clu protein. (a) Maximum Intensity Z-projection confocal images of LRRK2 KO and WT brains (13-months old mice) stained for Clu (green), GFAP (red) and nuclei are stained with DAPI (blue). Scale bar 20 μm. (b) Cell lysates of LRRK2 KO and WT primary astrocytes were subjected to immunoblotting using Clu and GAPDH antibodies. Data are representative of three independent experiments and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test for preClu (p = 0.0353) and one-sample t-test for intracellular cleaved Clu (p = 0.0017). (c) Medium from LRRK2 KO and WT primary astrocytes were subjected to immunoblotting using Clu antibody. Quantification of extracellular Clu is normalized to GAPDH protein of cell lysates. Data are representative of three independent experiments and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test; p = 0.0378