Fig. 2

LRRK2 G2019S-KI brain slices and primary astrocytes exhibited increased levels of Clu protein. (a) Maximum Intensity Z-projection confocal images of LRRK2 G2019S-KI and WT brains (13-months old mice) stained for Clu (green), GFAP (red) and nuclei with DAPI (blue). Scale bar 20 μm. (b) Cell lysates of LRRK2 G2019S-KI and WT primary astrocytes were subjected to immunoblotting using Clu and GAPDH antibodies. Data are representative of three independent experiments and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test; preClu: p = 0.0405 and Clu: p = 0.0257. (c) Medium from LRRK2 G2019S-KI and WT primary astrocytes were subjected to immunoblotting using Clu antibody. Quantification of extracellular Clu is normalized to GAPDH protein of cell lysates. Data are representative of three independent experiments and are expressed as the mean ± SEM. Data were analyzed using unpaired t-test; p = 0.0035