Fig. 2
From: GSK3 acts as a switch for transcriptional programs in a model of low-grade gliomagenesis
Reduced migration and altered morphology upon GSK3 inhibition. A Gene ontology analysis of intersected RNA-seq and mass spectrometry data demonstrates significant enrichment for wound healing, cell adhesion and ECM related terms among downregulated genes and proteins after GSK3-inhibition with CHIR99021. B Heatmap of selected candidates within the GO wound healing family. PDGFRA is among the significantly downregulated genes affecting migratory capacity. C After inflicting a scratch wound, CHIR99021-treated IDH1R132H astrocytes display decreased migration capacity over time, as shown in representative images. Wound width at different timepoints highlighted in grey (control) and light green (CHIR99021. Scale bar = 400 μm. Statistical analysis confirms that wound healing (migration) was significantly reduced after CHIR99021 treatment (non-linear regression curve analysis, p < 0.0001–0.0356) (n = 3 biological and 4 technical replicates each). D Immunofluorescence with DAPI (blue) and Phalloidin (red) staining demonstrates altered cell morphology upon GSK3 inhibition with reduced cell size and cell spreading. Scale bar = 20 μm. (n = 3 technical replicates). E Comparison of maximum cell diameters shows a significantly reduced cell diameter after treatment with CHIR99021 (p < 0.0001) (n = 3 biological replicates). F, G Simplified schematic of FAK/RhoA/ROCK interaction and corresponding heatmap of the respective genes upon modulation with CHIR99021