Fig. 3

Subcellular distribution of TRIM32 protein. (A) Localization of TRIM32-WT-EGFP and mutants in HEK-293T cell. HEK-293T cells were transfected with TRIM32-WT-EGFP, P130S-EGFP, 487N-EGFP, R394H-EGFP, V591M-EGFP and P619S-EGFP. (B) Co-localization of TRIM32-EGFP mutants and TRIM32-WT-pmCherry in HEK-293T cells. TRIM32-EGFP mutants and TRIM32-WT-pmCherry were transfected into HEK-293T cells. TRIM32-EGFP mutants protein can be found in aggregates that co-localize with the TRIM32-WT -pmCherry fluorescence, which appear yellow in the merged image. Scale bar = 15 µm (40 × magnification)