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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Correction: Investigating genotype-phenotype correlation of limb-girdle muscular dystrophy R8: association of clinical severity, protein biological function and protein oligomerization

Fig. 2

Interaction and ubiquitination detection between TRIM32 and its substrate. (A) Left panel: Flag-NDRG2 was immunoprecipitated from HEK293T cells expressing HA-ubiquitin and either TRIM32-WT, P130S, D487N, R394H, V591M or P619S. Immunoprecipitated NDRG2 was probed for the presence of conjugated TRIM32 and HA-ubiquitin by western blot. Right panel: Protein grey value analysis performed using ImageJ, and the data are presented as means ± SEMs. (B) Left panel: Flag-ULK1 was immunoprecipitated from HEK293T cells expressing HA-ubiquitin and either TRIM32-WT, P130S, D487N, R394H, V591M or P619S. Immunoprecipitated ULK1 was probed for the presence of conjugated TRIM32 and HA-ubiquitin by western blot. Right panel: Protein grey value analysis performed using ImageJ, and the data are presented as means ± SEMs. A high molecular weight smear is seen on the HA blot indicative of high ubiquitination. Student’s T-tests were performed to analyze the data and all data are shown as three biological replicates. “*” on the histogram columns represents the significant differences between each variant and WT; “*” on the horizontal line represents the significant differences of the variants below it. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance

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Acta Neuropathologica Communications

ISSN: 2051-5960

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