Fig. 4

RAB7 A genetic knockdown increases seeded tau aggregation across neuronal subtypes. a UMAP visualization of single cell RNA-seq (scRNA-seq) data generated from DIV14 hiPSC-neurons colored by protocol (top) or by RAB7 A expression (bottom). b Representative DIV44 images from SI-AD seeded hiPSC-neurons pre-treated with non-targeting control (NTC) siRNA or RAB7 A siRNA. MC1 labels tau aggregates; MAP2 labels dendrites. Scale bar = 50 µm. c Quantification of MC1-positive spot area normalized to MAP2 area in hiPSC-neurons seeded with 0.5 nM SI-AD following treatment with non-targeting control (NTC) or either of two individual (non-pooled) RAB7 A siRNA. Data normalized to cortical inhibitory hiPSC-neurons treated with NTC siRNA. Data represents the mean + SD (3 technical replicate wells, 20 fields per well). ns = not significant, * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001, **** = p-value < 0.0001 according to two-way ANOVA with Tukey’s test