Fig. 2

Distinct hiPSC-derived neuronal subtypes show differential vulnerability to seeding with AD brain-derived Tau pathology. a Representative DIV44 images from hiPSC-neuronal subtypes seeded with 1 nM SI-AD. MC1 labels Tau aggregates; MAP2 labels dendrites. Scale bar = 50 µm. b Quantification of MC1-positive spot area normalized to soluble Tau (HT7) area at DIV21 in 5 hiPSC-neuron subtypes across 5 donor lines, seeded with range of SI-AD concentrations. Data normalized to cortical inhibitory hiPSC-neurons seeded with 1 nM SI-AD. Data represents the average of 4 or 5 biological replicates + SD (3 technical replicate wells, 30 fields per well). ns = not significant, * = p-value < 0.05, ** = p-value < 0.01, *** = p-value < 0.001, **** = p-value < 0.0001 according to one-way ANOVA with Tukey’s test. c Representative DIV44 images from unseeded hiPSC-neurons (left) or hiPSC-neuronal subtypes seeded with 1 nM SS-AD. MC1 labels tau aggregates; MAP2 labels dendrites. Scale bar = 50 µm. d Quantification of MC1-positive spot area normalized to soluble Tau (HT7) area at DIV21 in 5 hiPSC-neuron subtypes across 3 donor lines, seeded with range of SS-AD concentrations. Data normalized to cortical inhibitory hiPSC-neurons seeded with 1 nM SI-AD. Data represents the average of 2 or 3 biological replicates + SD (3 technical replicate wells, 30 fields per well). ns = not significant, ** = p-value < 0.01 according to one-way ANOVA with Tukey’s test