Fig. 4

Replication of mouse-adapted sCJD MM1 prions in deer mouse whole brain slice cultures. a Deer mouse cerebellar (CSC) and whole brain slice cultures (WBSC) were prepared and seeded with either human (MM1-HU) or mouse (MM1-DM, third passage) sCJD-MM1 brain homogenates. b PrP^Sc seeding activity was monitored bi-weekly for 98 days post-inoculation using RT-QuIC. c Proteinase K-resistant PrP^Sc was not detected after 98 days in whole brain slice cultures seeded with deer mouse-passaged sCJD MM1 prions. d Additional infections were performed with deer mouse cerebellar slice cultures seeded with human sCJD VV2 (VV2-HU) and deer mouse whole brain slice cultures seeded with various prion strains: human sCJD VV2, rodent-adapted sCJD MM1, human sCJD MM1, and human VPSPR (VPSPR-HU). e PrP^Sc seeding activity in each group was tracked over 168 days post-inoculation with RT-QuIC. f Proteinase K-resistant PrP^Sc was not detected after 168 days in whole brain slice cultures seeded with deer mouse-passaged sCJD MM1 prions