Fig. 6
From: Premature skeletal muscle aging in VPS13A deficiency relates to impaired autophagy
The absence of Vps13A promotes impaired autophagy, exacerbating muscle aging phenotype. (a) Western-blot (Wb) analysis with specific antibodies against LC3-I/II, ATG5, ATG7, RAB3, LAMP1, LAMP2 and P62 in quadriceps lysates from Vps13a-/- and WT female mice aged 8 months. GAPDH, loading control. Right, densitometric analysis. Means ± SEM (n = 6); *p < 0.002, unpaired Student t-test. (b) Western-blot (Wb) analysis with specific antibodies against LC3-I/II and LAMP1 in quadriceps lysates from Vps13a-/- and WT female mice aged 8 months, ad libitum fed or starved for 24 h. GAPDH, loading control. Below, densitometric analyses. Means ± SEM (n = 4); **p < 0.002, paired Student-t-test. (c) Western-blot (Wb) analysis with specific antibody against NCAM1 in quadriceps lysates of Vps13a-/- and WT female mice aged 8 months. GAPDH, loading control. Below, densitometric analysis. Means ± SEM (n = 6); **p < 0.002, unpaired Student t-test. (d) Western-blot (Wb) analysis with specific antibodies against NCAM1, LAMP-1 and P62 in quadriceps lysates of 8-month-old Vps13a-/- or WT female mice treated with rapamycin (2 mg/kg, intraperitoneally injected every 24 h for 5 days). GAPDH, loading control. Right, densitometric analyses. Means ± SEM (n = 6); **p < 0.002, unpaired t-test. (e) Western-blot (Wb) analysis with anti-NCAM1 antibody of lysates from muscle biopsies of patients with VPS13A disease (Dis.; P) and control. GAPDH, loading control. Right, densitometric analysis. Means ± SEM (n = 3); *p < 0.002, paired Student t-test.