Fig. 3
Histopathological analysis of de novo tumors. De novo tumor generation for (A) diffuse midline glioma through midline injection, 49 days post-injection, and (B) supratentorial high-grade glioma through cortical injection, 50 days post-injection. (A-B) Whole brain tissue section and tumor stained with H&E. Higher magnification of inset images reveal (i) infiltrative tumor cells, (ii, arrows) microvascular proliferation, and (iii, asterisk) pseudo-palisading necrosis. (C). Flow cytometric analysis of IL13RA2 and PDGFB expression in cell lines generated from the midline and cortical de novo tumors. (D) Survival analysis of orthotopically injected cell lines in the midline and cortical location of Nestin-Tva; p53fl/fl mice. (E) Histological validation of PTEN expression, PTEN loss, and H3K27me3 loss in de novo tumors derived from p53fl/fl/PTENwt/wt, p53fl/fl/PTENfl/fl, and p53fl/flPTENfl/flH3.3K27Mmut mice, respectively. (F) Suvival comparison between p53fl/fl, p53fl/flPTENfl/fl, and p53fl/flPTENfl/flH3.3K27Mmut mouse models