Fig. 4

Knockdown of essential SRSF7 reduces levels of STMN2, though not TDP-43, and impairs axon regrowth in induced neurons. A Representative immunoblot of STMN2, TDP-43, beta-tubulin IIIb (Tuj1) from induced neurons following depletion of SRSF7 or a non-targeting control. B Quantification of the levels of STMN2, TDP-43, and Tuj1 normalized to GAPDH, from three independent experiments (n = 7–8, *** = p < 0.001, two-tailed t-test). C Experimental workflow for axotomy assay. D Representative images of neurite regrowth following transfection of siRNAs targeting STMN2, SRSF7, or non-targeting control. E Axotomy assay with quantification of neurite regrowth from three separate devices for each condition (one-way ANOVA, **** = p < 0.0001). F Experimental workflow for rescue axotomy assay. G Axotomy assay of neurons transduced with lentivirus expressing STMN2-GFP compared to GFP control following siRNA transfection of non-targeting control or SRSF7 siRNA. Quantification of neurite regrowth, results from multiple separate devices for each condition (one-way ANOVA, **** = p < 0.0001)