Fig. 1

Poly-PR decreases STMN2 expression in induced neurons and impairs axon regrowth. A Assessment of STMN2 and TARDBP relative expression via qPCR in human induced neurons transduced with lentivirus expressing GFP-(PR)₅₀ or GFP only and then collected 6 days later. (** = p < 0.01, two-tailed t-test; n = 4 from two independent differentiations). B Schematic of axotomy assay in induced neurons in microfluidics chambers. C Representative fluorescent images of neurite regrowth of induced neurons transduced with lentivirus for GFP-(PR)₅₀ or GFP only. D Quantification of neurite regrowth from three devices for each condition. (GFP-(PR)₅₀ n = 147, GFP n = 108; **** p < 0.0001, two-tailed t-test)