Fig. 4
From: TRPA1 exacerbates selective retinal ganglion cell vulnerability under acute ocular hypertension
HC showed protective effect on RGC. (A) Experimental design. (B) TUNEL staining of retinal slices showing decreased apoptosis after intravitreal delivery of 100 \(\:{\upmu\:}\)M HC. Scale bar: 25 μm. (C) Retinal flatmounts showing RGCs and their axons under control, AOH, and AOH + HC conditions. Scale bar: 50 μm. (D) Relative RGC counts and (E) RGC axon density calculated in the CTB-labeled retina in the center and periphery (n = 4 for each group, paired t-test, **P < 0.02, ***P < 0.01). (F) Track plots of AOH mice treated with PBS or (G) HC in the open field. (H-K) Comparison of (H) total distance travelled, (I) ratio of central to peripheral distance travelled, (J) percentage of total immobile episodes, and (K) number of zone cross between PBS and HC-treated AOH mice (n = 6 for each group, unpaired t-test, *P < 0.05, **P < 0.02, ***P < 0.01). Results are presented as mean ± standard deviation (SD). AOH, acute ocular hypertension