Fig. 3

Imaging, histological and pathological features of the calcified PCPs. A: Images from calcified PCP patients. The yellow arrows in the CT images indicate calcification of the tumors. The MR images showed the solid components and cystic cavities of the tumors. The ACP, as a control, showed calcification (yellow arrow), solid components and cystic cavity in the tumor. B: Histological and pathological features of the calcified PCPs. Calcified PCP Patient 1: the green arrows in HE staining indicate wet keratins. Pan-CK was localized to the cytoplasm, beta-catenin was localized to the membrane, and the BRAFv600e mutation was positive. Calcified PCP Patient 2: the white arrow in HE staining indicates calcification. Pan-CK was localized to the cytoplasm, and beta-catenin was localized to the cell membrane, but the BRAFv600e mutation was negative. Calcified PCP Patient 3: the white arrow in the HE image indicates ossification. Pan-CK was localized to the cytoplasm, and beta-catenin was localized to the cell membrane, but the BRAFv600e mutation was negative. Calcified PCP Patient 4: the white arrow in the HE-stained image indicates calcification. Pan-CK was localized to the cytoplasm, and beta-catenin was localized to the membrane rather than the nucleus, but the BRAFv600e mutation was negative. In the ACP patient, as a control, the ACP showed cluster cells (yellow arrow), wet keratin (green arrow) and ossification (white arrow) on HE staining. Pan-CK was localized to the cytoplasm, and beta-catenin was localized to the nucleus rather than the cell membrane, but the BRAFv600e mutation was negative