Fig. 3 

Precise matching across continuous sections (Top) Images of immunofluorescence (1A/3A) and EM (2A) from three continuous sections demonstrate accurate alignment of αS inclusions in a single cell, aided by DAPI-stained nuclei. (Middle) Whole-section views confirm alignment between three continuous sections (1/2/3). Framed fields are respectively enlarged into corresponding images on top and at bottom. (Bottom) The left two enlarged images (1B/2B) highlight αS inclusions and nuclei as fiducial markers. Arrows and arrowhead of the same color respectively denoted the αS inclusions and nuclei in the same cells on different section. Note: the white asterisk pointed at a folded area occasionally occurring on the 70 nm section, which should be avoided for CLEM analysis. A sketch map showing the thickness of three continuous sections is on the right. Scale bars are included in each individual image