Fig. 5

Intrahippocampal expression of sIl10R and sIl4R prevents acceleration of amyloid deposition in aged TgCRND8 mice. 6-month-old TgCRND8 mice were stereotaxically injected in the hippocampus with AAV-sIl10R and AAV-sIl4R and aged to 9 months of age. AAV-GFP was used as control. A-D. Representative sections showing pan-Aβ (A) and Thioflavin S cored Aβ deposits (C) staining. Scale, 250 μm. Quantification of the forebrain amyloid immunostaining intensity burden and Thioflavin S stained cored plaque count in three non-consecutive sections represented by mean ± standard error of the mean (B, D). 1-way ANOVA, *p < 0.001. E-G. FA-, SDS- and RIPA-extracted Aβ42 and Aβ40 levels were detected by ELISA and plotted as scatter dot plot of mean ± standard error of the mean. Aβ42 and Aβ40 levels were quantified with 1-way ANOVA; **p < 0.01, ***p < 0.001, ****p < 0.0001. H-K. Representative sections showing Iba-1 (H) and GFAP (J) staining. Scale, 250 μm (main panel), 50 μm (inset). Iba-1 and GFAP immunohistochemistry burden was quantified using ImageScope analysis (I, K). 1-way ANOVA, *p < 0.001. 1-way ANOVA; **p < 0.01. n = 5 mice/group