Fig. 4

sIl4R delays amyloid deposition in young TgCRND8 mice

Neonatal TgCRND8 mice were injected in the ICV with AAV-sIl4R (1E13 genomes/ml, 2 µl per ventricle) and brains were harvested at 3 months of age. Control mice were injected with saline. A-B. Representative sections showing pan-Aβ (A) staining in the cortex and hippocampus of Tg mice. Scale, 250 μm. Forebrain plaque count in three non-consecutive sections represented by mean ± standard error of the mean (B). Student’s two-tailed t test; ***p < 0.001. n = 11–12 mice/group. C-E. FA-, SDS- and RIPA -extracted Aβ42 and Aβ40 levels were detected by ELISA and plotted as scatter dot plot of mean ± standard error of the mean. Aβ42 and Aβ40 levels were quantified with 1-way ANOVA; *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001. n = 11–12 mice/group. F-I. Representative sections showing Iba-1 (F) and GFAP (H) staining in the cortex and hippocampus of Tg mice. Iba-1 and GFAP immunohistochemistry burden was quantified using ImageScope analysis of staining intensity (G, I). Scale, 250 μm (main panel), 50 μm (insets). Student’s two-tailed t test; ***p < 0.001. n = 11–12 mice/group. Student’s two-tailed t test; **p < 0.01