Fig. 2

sIl10R overexpression attenuates amyloid deposition in young TgCRND8 mice. Neonatal TgCRND8 mice were injected in the cerebral ventricles with AAV-sIl10R (1E13 genomes/ml, 2 µl per ventricle) and brains were harvested at 3 months of age. Naïve age-matched mice were used as control. A-D. Representative sections showing pan-Aβ (A) and Thioflavin S (C) staining in the cortex of Tg mice. Scale, 50 μm. Quantification of the immunostaining intensity of amyloid plaques (B) and ThioS stained cored plaque counts (D) represented by mean ± standard error of the mean. Student’s two-tailed t test; *p < 0.05. E-G. FA, SDS and RIPA extracted Aβ42 and Aβ40 levels were detected by ELISA and plotted as scatter dot plot of mean ± standard error of the mean. 1-way ANOVA; *p < 0.05. n = 16 mice/group (A-D) and n = 8 mice/group (E-G). H-K. Representative sections showing Iba1 (H) and GFAP (J) staining in the cortex of TgCRND8 mice. GFAP and Iba-1 immunoreactivity burden was quantified using ImageScope analysis of staining intensity (I, K). Scale, 50 μm. Student’s two-tailed t test; **p < 0.01. n = 16 mice/group