Fig. 6

Integrated analysis of gene expression and DNA methylation. (A) Venn diagram illustrates the overlap of differentially expressed genes with hyper- or hypomethylated status. (B) Depicts a heatmap displaying differentially methylation patterns of the 14 genes that are overlapping DMT and DEG. Matrix represents the m6A percentage 0 = 0% to 5 = 1200%. (C) Demonstrates a network diagram featuring key genes (depicted as green circles) with hyper- or hypomethylated status, their gene co-expressions (indicated by red lines), or protein-protein interactions (indicated by purple lines). These genes are associated with the top eight GO pathways (depicted as pink circles) and KEGG pathways (depicted as blue circles). Additionally, a node representing another gene (depicted as a green circle with a red border) not included in our primary analysis is shown, which typically exhibits co-expression with PPIF. All pathways present p-value < 0.05. Venn diagram showing the hub genes between DMT and (D) Magenta, (E) Purple, and (F) Lightcyan module