Fig. 3
From: The role of plasma metagenomic sequencing in identification of Balamuthia mandrillaris encephalitis
B. mandarallis trophozoites and cysts, and free-living amoeba PCR. a Trophozoites adjacent to cerebellar granule cells (Giemsa, 40x). High-power views of b trophozoites (H&E, 100x) and c cysts (H&E, 100x). d PCR amplification curves of B. mandrillaris specific nucleic acid in: (A) B. mandrillaris positive control (Cp = 28.3) and (B) patient’s cerebellar FFPE tissue (Cp = 31). (C) negative controls (Naegleria fowleri and Vero cell line controls). Scale bars: a = 200 µm; b, c = 10 µm