Fig. 2

Generation of Tdp-43 knock-in mouse model. (A) Illustration of the target allele design for the murine Tardbp gene. (B) Genotyping PCR identifies a wild-type and a heterozygous littermate where the band size of 351 bp indicates the presence of the floxed target allele. (C) Schematic of the double-heterozygous Cre^±:Tdp-43∆NLS^± strain generation. (D) Illustration of the Cre-mediated recombination of floxed WT and mutant Exon-3 deletion and re-orientation, respectively, resulting in the expression of mutant Tdp-43∆NLS variant in the desired cell type in the central nervous system (CNS)