Fig. 5

A Comparison of Pilocytic Astrocytomas to Pediatric HGG H3 K27M Gliomas Demonstrates Enrichment in Glucose in the H3 Gliomas but No Difference in Acylcarnites. a and b Heatmap reporting the relative abundance of significantly enriched (FDR < 0.05) polar (a) and lipid (b) metabolites in pilocytic astrocytomas (PA) or pediatric high grade glioma H3K27 mutant tumors (Peds H3K27M) after normalization of batch corrected data. Metabolites presented in order of hierarchal clustering results (left). Samples are grouped by subtype (bottom). The scale bar (right) denotes the Z-score relative to the mean for each metabolite. c and d PCA of normalized batch corrected polar (c) and lipid (d) metabolite data. Graphs created using PCA1 and PCA2 (c) or PCA3 (d). PA samples are depicted in blue and Peds H3K27M samples are depicted in red. e and g Volcano plot depicting significance (-log₁₀(p-value) and relative abundance in PA versus Peds H3K27M samples (log₂FC) for each polar (e) or lipid (g) metabolite. Dashed lines denote cut-offs for significance (horizontal, FDR < 0.05) and enrichment (vertical, log₂FC > 0.5 or < -0.5 for polar metabolites and > 2 or < -2 for lipid metabolites). Metabolites or transcripts with significance and enrichment are in red, significance only in blue, enrichment only in gray, and neither in black. Purple in (g) denotes acylcarnitines. (f) Relative abundance of glucose in individual PA (blue) and Peds H3K27M (red) samples using the normalized batch corrected data. *** denotes p < 0.001. Analysis of metabolite data was done using batch corrected data normalized across only PA and Peds H3K27M samples