Fig. 2

Pediatric Gliomas are Enriched in Glucose Whereas Adult Tumors have Hallmarks of a Hypoxic Microenvironment. a, b, d–g Relative abundance of glucose (a), acetylcarnitine (b), 3-hydroxybutyrate (d), lactate (e), and 2-hydroxyglutarate (f and g) in individual adult (blue) and pediatric tumors (Peds, red) using the batch corrected data normalized across all samples. ** denotes p < 0.01, *** denotes p < 0.001. c, i Volcano plot depicting significance (-log₁₀(p-value) and relative abundance in adult versus pediatric samples (log₂FC) for each polar metabolite (c) or metabolic transcript (i). Dashed lines denote cut-offs for significance (horizontal, FDR < 0.05) and enrichment (vertical, log₂FC > 0.5 or < -0.5 for polar metabolites and > 2 or < -2 for metabolic transcripts). Metabolites or transcripts with significance and enrichment are in red, significance only in blue, enrichment only in gray, and neither in black. Purple in (i) denotes GLUT transporters. h Output of GSEA analysis for Buffa Hypoxia Metagene. Input is ranked fold change of transcripts in adult relative to pediatric gliomas. The green line denotes enrichment score along the ranked gene set. Black lines denote locations of genes in the gene set within this ranked list. FDR values are indicated