Fig. 5

Protein interaction networks identified in PD and MSA brain. BAR identified proteins were analyzed with STRING to plot known functional interactions for each proximal proteome. Proteins were grouped according to whether they were unique to PD/DLB, MSA, or shared between synucleinopathies. STRING networks for A BAR-PSER129 and B BAR-MJFR1 are shown. CentiScaPe 2.2 was used to determine the central “driver” node for each STRING network (i.e., highest “betweenness” score). The driver node was enlarged, and the first neighbors in network highlighted. For, BAR-PSER129 C and BAR-MJFR1 D MCL clustering was used to group nodes. Clusters with 6 nodes or more are depicted. Top enrichment (GOCC, GOMF, GOBP) are annotated onto each cluster. E Physical interaction network of proteins specific to MSA for all differential expression analysis. BAR target SNCA was included in the network. Nodes annotated with top enriched pathway and disconnected nodes not shown