Fig. 2

BAR capture in the PD/DLB and MSA brain. A 10 μg tissue lysate protein for each case were separated on 4–12% Bis–Tris gel transferred onto PVDF and stained for total protein (Revert Total Protein Stain, LI-Cor), PSER129, or αsyn. Chemiluminescence was used to detect PSER129 and αsyn. B PSER129 and αsyn relative density values were first normalized to total protein (i.e., loading control) and then normalized to the mean intensity for each group. C Spot blots of 1 µl eluent from BAR captures, including BAR-NEG (Primary antibody-omission control, “-”), BAR-PSER129 (aggregates “P”), and BAR-MJFR1 (total αsyn “M”) for each synucleinopathy case. Blots were probed for either biotin (ABC reagent) or αsyn (BDSYN1). D BAR enrichment was calculated by dividing for “P” and “M” by the relative density value for “-” (i.e., fold-enrichment over background) (mean ± SEM, Sidak's multiple comparison,**p-adj. < 0.005, n = 5–10)