Fig. 5

Accumulation of deiminated proteins in neurons, especially in damaged neurons in the post-ischemic brain Coronal brain sections were prepared from sham controls (A) as well as the cortical penumbra (B-F, H, I) of the MCAO group at 12 h (B-D) or 24 h (E, F, H, I) post-MCAO. Coronal brain sections were further prepared from the cortical penumbra of the MCAO + BBCA group at 24 h (F, I) post-MCAO. Triple immunofluorescence staining was conducted using the anti-F95 antibody, anti-NeuN antibody, and DAPI (A-F) or with the anti-F95 antibody, TUNEL, and DAPI (H, I). (G) The ratio of F95- to NeuN-positive cells is presented (n = 12 from 3 animals). (J) The ratio of TUNEL-positive cell to F95-positive cells is presented (n = 12 from 3 animals). Arrows indicate F95 immunoreactivity localized in the neuron, while asterisks indicate localization of F95 immunoreactivity in degenerating neurons. Arrowheads highlight F95 immunoreactivity in non-neuronal cells, and double arrowheads highlight F95 immunoreactivity in vasculature. Double asterisks in H and I indicate localization of F95 immunoreactivity in TUNEL-positive cells. Scale bars represent 50 μm. **p < 0.01, ***p < 0.001 compared to the sham group and ^##p < 0.01, ^$p < 0.05 between indicated groups