Fig. 3

PAD4 expression in the different brain cell types in the post-ischemic brain (A) Schematic representation of the brain region bordering the infarct core and penumbra. (B-D1) Coronal brain sections were prepared from sham controls (B) as well as the cortical penumbra (C, C1, C2) and cortical core (D, D1) of the MCAO group at 24 h post-surgery. Triple immunofluorescence staining was performed with anti-PAD4 antibody, anti-NeuN antibody, and DAPI. Arrows indicate PAD4 immunoreactivity in neurons and arrowheads indicate PAD4 immunoreactivity in non-neuronal cells. Asterisks depict PAD4 immunoreactivity in degenerating cells or cell debris. Scale bars represent 50 μm