Fig. 6
Composite schematic depiction of phosphorylated alpha-synuclein (p-aSyn, S129) immunofluorescence detection in the brains of M83+/- mice. A Average p-aSyn (S129) fluorescence signal (percentage of area) in the regions is highlighted by colored circles as follows: small circle (0.05–0.2%), medium circle (0.2–1%) and large circle (1–5%). Also see Supplementary Figure S4 for the low magnification (2X) panoramic views annotating select brain regions and S7B. Regions shown in 6A: (view in S4, Pons) 
gigantocellular nuclei (GRN), 
pontine reticular nuclei (Rt), 
vestibular nuclei; 
deep cerebellar nuclei; (view in S4, Midbrain) 
substantia nigra, 
red nucleus, 
periaqueductal grey matter; (view in S4, Thalamus) 
medial and lateral hypothalamus, 
subthalamic nucleus, 
ventromedial and ventrolateral thalamic nuclei. B Quantitative estimates of p-aSyn S129 immunopositivity (immunofluorescence signal intensity from two serial sections) in the GRN/Gi in cohorts of M83+/- mice, expressed as percentage of the area (total average area/image: 625,000 um2) in 10X views (see Fig. 5A–C). C Quantitative estimates of the total number of neurons (NeuN immunofluorescence) in of GRN/Gi from cohorts of M83.+/- mice. Statistics in Fig. 6B–C: One-Way ANOVA followed by Tukey’s multiple column comparisons (PBS, n = 5; monomeric aSyn, n = 5; PFF aSyn DPI-30 and DPI-60, n = 3 and PFF aSyn DPI ≥ 108, n = 5; ****p < 0.0001; Error bars, Mean ± SD). (In Fig. 6B) Black asterisks represent significant differences between PBS and PFF aSyn-injected cohorts, while the grey asterisks indicate significant differences between monomeric aSyn and PFF aSyn-injected cohorts. Only significant differences are highlighted (Fig. 6B–C)