Fig. 4
Measurements of sensorimotor behaviors in cohorts of heterozygous M83+/- mice. A–B Assessment of motor coordination of M83+/- cohorts (injected with PBS, monomeric aSyn or PFF aSyn) in the balancing beam test over a longitudinal period (shown on x-axis as DPI, days post-injection). The line graphs show the traversal time (seconds), number of slips and speed (m/s) on 8 mm wide beam (in 4A) and 16 mm wide beam (in 4B). C Representative images showing postural adaptations and performance on 8 mm beam by PBS or PFF-injected M83+/- mice. D Bar graphs depicting performance of M83+/- cohorts (injected with PBS, monomeric aSyn or PFF aSyn) in the pole test with individual points representing the quantitative measurement of the turning time, traversal time and total time on pole at DPI-60 and DPI-120 (DPI, days post-injection). E Assessment of motor coordination of M83+/- cohorts (injected with PBS, monomeric aSyn or PFF aSyn) in the rotarod test over a longitudinal period (shown on x-axis as DPI, days post-injection). The line graphs depict the quantitative measures of the latency to fall (time, in seconds) and speed at fall (rotations per minute, RPM). F Postural reflex assessment in modified tail-suspension test with bar graphs depicting hindlimb clasping by cohorts of M83+/- mice (injected with PBS, monomeric aSyn or PFF aSyn) at DPI-60 and DPI-120 (DPI, days post-injection). G–H Assessment of thermal nociception (hot plate, in 4G) and mechanical allodynia (von Frey, in 4H) in cohorts of M83+/- mice (injected with PBS, monomeric aSyn or PFF aSyn). Statistics in Fig. 4A-H: One-Way ANOVA followed by Tukey’s multiple column comparisons (PBS, n = 5; monomeric aSyn, n = 5 and PFF aSyn, n = 6–9; *p < 0.05, **p < 0.01; Error bars, Mean ± SD). Black asterisks (all panels) represent significant differences between PBS and PFF aSyn-injected cohorts, while the grey asterisks (in 4A, 4E and 4G) indicate significant differences between monomeric aSyn and PFF aSyn-injected cohorts. Only significant differences are highlighted