Fig. 5

Differentially expressed miRNAs (DEmiRNAs) between CHRNE-patients, other CMS subtype patients and healthy controls. Volcanos and heatmaps of the top DEmiRNAs based on the adjusted p value (< 0.05) for (a) CHRNE-patients versus controls and (b) CHRNE-patients versus other CMS subtypes. In heatmaps, the CHRNE group is shown in red colour, the control group (healthy individuals) is shown in blue colour and the other CMS subtype group in yellow. The colour key panel shows the Z-score values calculated for each miRNA, by subtracting the row-mean and then dividing by the standard deviation. Z-scores describe the expression of each miRNA in relation to the mean. Overexpressed miRNAs are shown in red, underexpressed miRNAs in blue. White colour indicates expression change close to 0. Hierarchical clustering was performed for samples and miRNAs. (c) Comparison of CHRNE-patients and other CMS subtypes versus controls revealed only miR-4433b-3p to be differentially expressed. (d) Venn diagram showing the number of unique and common miRNAs that reached significace in the three comparisons, CHRNE-patients versus controls, CHRNE-patients versus other CMS subtypes, CHRNE-patients and other CMS subtypes versus controls