Fig. 7

Transcriptomic and proteomic changes in LCA5 retinal organoids. (A-D) Transcript changes in LCA5 KO retinal organoids (ROs) compared to isogenic control (WT) ROs. (A-C) Volcano plots showing the significant DE genes in (A)WT to LCA5 KO D200 ROs, (B) eupatilin treated, and (C) fasudil treated ROs compared to WT following removal of vehicle-associated changes, significant genes highlighted in red. (D) Heatmap of top 50 DE genes from WT vs. LCA5 KO and their expression in treated organoids, * indicates changes that are significantly different to untreated comparisons. (F-G) Proteomic analyses of LCA5 ROs. Comparison of DE proteins between WT and LCA5 KO (E) and WT and LCA5 JB342 (F). (G) Correlation of DE proteins between control and LCA5 KO (y-axis) and LCA5 JB342 (x-axis), significantly DE proteins highlighted in green