Fig. 2

Loss of LCA5 causes a distinctive ciliary phenotype in LCA5 KO retinal organoids. Representative images of isogenic control and LCA5 KO unfixed retinal organoids at D200, as indicated, stained for A) LCA5 (magenta) and the basal body marker PCN (yellow) B) LCA5 (magenta) and axoneme marker Arl13b (yellow) C) CEP290 (magenta) and Arl13b (yellow); D) IFT88 (magenta) and PCN (yellow). DAPI was used as nuclear staining marker (cyan). Scale bars 10 μm. Inset boxes show cilia at higher magnification. Scale bar 5 μm