Table 2 Immunohistochemistry panels for analysis of microglia changes in the human ALS brain
PANEL 1 Quantification panel | |||
|---|---|---|---|
Primary antibody, Concentration | Company, Catalogue number | Secondary antibody, Concentration | Company, Catalogue number |
Rabbit IgG anti-pTDP-43 (Ser 409/410), 1:3,000 | Cosmobio, TIP-PTD-P02 | Goat anti-rabbit AlexaFluor® 488, 1:500 | Jackson Laboratories, 111-545-144 |
Mouse IgG1 anti-HLA-DR, 1:1,000 | DAKO, M0775 | Goat anti-mouse IgG1 AlexaFluor® 546, 1:500 | Thermo Fisher Scientific, A21123 |
Mouse IgG3 anti-CD68, 1:200 | Abcam, ab783 | Goat anti-mouse IgG3 AlexaFluor® 594, 1:500 | Thermo Fisher Scientific, A21155 |
Guinea pig IgG anti-Iba1, 1:2,000 | Synaptic Systems, 234-004 | Goat anti-chicken AlexaFluor® 647, 1:500 | Thermo Fisher Scientific, A21450 |
PANEL 2 Confocal panel | |||
Primary antibody, Concentration | Company, Catalogue number | Secondary antibody, Concentration | Company, Catalogue number |
Chicken IgY anti-Iba1, 1:1,000 | Synaptic Systems, 234-009 | Goat anti-chicken AlexaFluor® 488, 1:500 | Thermo Fisher Scientific, A11039 |
Rabbit IgG anti-CD68, 1:1,000 | Abcam, ab215212 | Goat anti-rabbit AlexaFluor® 594, 1:500 | Jackson Laboratories, 115-585-166 |