Fig. 6

Microglia may trigger oxidative stress by producing ROS in the RIBI model. (A-C) Quantification of MDA content, SOD activity, and CAT activity in the brain tissues to evaluate the level of oxidative stress after whole-brain radiation. (D-F) The gating strategy (D) and flow cytometry plots (E) to indicate the dynamic changes in the ROS content within microglia (CD45⁺CD11b⁺TMEM119⁺) after RIBI modeling. The FMO negative control consisted of CD45⁺CD11b⁺TMEM119⁺ cells from a sample in which only the DCFH-DA fluorescence probe was omitted. 1 d, 1 day. 3 d, 3 days. 7 d, 7 days. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham. n = 5 per group