Fig. 5

Inhibiting SUR1-TRPM4 with GLB ameliorates brain atrophy, cognitive impairment and neuropathological damage in RIBI model. (A) The MRI scanning results to evaluate the severity of brain atrophy and the volume of lateral ventricles of indicated groups 8 weeks post-radiation. (B-D) The effect of blocking SUR1-TRPM4 on post-brain radiation spatial memory and learning deficits assessed by Morris Water Maze analysis 8 weeks post-radiation, including the mean latency and distance to reach the hiding platform (B), and the frequency of crossing the platform area (C) during the probe trial (D). (E-G) The effect of blocking SUR1-TRPM4 on post-brain radiation neuropathological damage characterized by the changes in immunofluorescent staining for Iba-1 in hippocampal DG region and cortex (E), DCX in hippocampal DG region, and GFAP in hippocampus (F). Scale bar = 100 μm. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus wild type sham; #P < 0.05, ##P < 0.01, ###P < 0.001 versus wild type vehicle. n = 5 or 10 per group