Fig. 4

SUR1-TRPM4 mediates the activation of NLRP3 inflammasome in BV2 cells challenged with irradiation. (A) Confocal analysis of TRPM4 and SUR1 staining in radiation-primed BV2 cells. (B) The association of NLRP3 and caspase-1 detected by Co-IP, in radiation-primed BV2 cells with or without ATP. Samples are immunoprecipitated with isotype IgG as a control. (C-D) The immunofluorescence reflecting the caspase-1 activity in radiation-primed BV2 cells challenged with ATP with or without MCC950. (E-F) The protein expression levels of caspase-1 p20 and supernatant IL-1β reflecting the NLRP3 activation in radiation-primed BV2 cells challenged with ATP with or without MCC950. (G-H) Western blotting results manifesting the levels of NLRP3 inflammasome activation in radiation-primed BV2 cells challenged with ATP, in the presence of indicated SUR1 or TRPM4 inhibitors. Rad, radiation. Scale bar = 50 μm. Data are represented as mean ± SD. ***P < 0.001 versus control. ###P < 0.001 versus radiation-primed BV2 cells challenged with ATP. n = 3 per group