Fig. 4
From: Tau oligomers impair memory and synaptic plasticity through the cellular prion protein
Time course of TauO binding and internalization in naïve and PrPC-overexpressing HEK293 cells. a Representative images of TauOs (green) in naïve (HEK293, upper panels) or PrP-overexpressing (HEK293-PrP, lower panels) cells after 15, 30 and 60 min of incubation (0.5 µM). TauOs immunofluorescence is detectable at early time points only in HEK293-PrP cells, indicating that PrPC facilitates the association of TauOs to cells. Scale bar 50 µm. b Higher magnification confocal images of TauOs (green) and PrPC (red) acquired after 60 min of incubation, showing TauOs—PrPC co-localization in naïve (b1) or PrP-overexpressing (b2) HEK293 cells. The co-localization channels (yellow) together with TauOs and PrP was reconstructed as 3D rendering and shown in (b3) and (b4) for both naïve and HEK293-PrP cells respectively. Major thick 10 µm