Fig. 1

Derivation of B16 melanoma variants by serial intracarotid artery injection. B Images from the surgical procedure in which B16 cells are delivered through a polyethylene tube inserted into the carotid artery, C chemiluminescence images of the five 3rd generation B16-B2 animals (B2:3:1, B2:3:2, B2:3:3, B2:3:4, B2:3:5) showing tumor growth in the head, D H&E sections of brain and lung from two 4th generation animals showing metastatic B16 tumor cells in both organs (arrows point to the locations of the higher power insets), A schematic outline of the derivation of the B16 melanoma variants which entailed recovering B16 cells from brain tissue and growing them for four successive generations. The final designations of the 4th generation cell lines that were used for RNAseq are shown