Fig. 1

Generation of the ANXA11-P36R knock-in mouse. a The genome structure of mouse Anxa11. The mouse Anxa11 P35 corresponds to human P36. The mutant nucleotide was labelled in red. The boxed sequences corresponded to guide RNA (gRNA) sequences. The underlined sequence is the PAM site (bold upper case). A synonymous mutation was introduced by replacing C with T (the shadowed case) to prevent secondary editing. b Alignment of amino acid in ANXA11 around mutant position is highly conserved in different species from human to zebrafish. Human ANXA11 sequences were used as a reference and the P36 residue is labelled with asterisk. c Sanger sequencing of wildtype and knock-in mice. Red triangle arrow showed the mutated residue 104 in wild-type (Wt), heterozygous (+ /R) and homozygous (R/R) ANXA11 mutant mice. Grey triangle arrow showed the created synonymous mutation preventing secondary splice by Cas 9. d Expression of Anxa11 mRNA level in mouse brain. The mRNA expression in both heterozygous and homozygous ANXA11-P36R knock-in mice was comparable with that in the wild-type