Fig. 2

Dopamine (DA) levels are decreased in the midbrain and striatum of two mouse models of JMJD3 deficiency. Brain tissues from the frontal cortex (CTX), striatum (STRM), midbrain (MB), cerebellum (CB) and medulla oblongata (MO) were micro-dissected, homogenized and the contents of DA and norepinephrine (NE) were measured by high performance liquid chromatography-mass spectrometry. (A, B) 4-month-old male conditional knockout (cKO) mice and their control littermates (WT). (C, D) 4-month-old male C57BL/6 mice underwent 5 consecutive days of intraperitoneal injection of GSK-J4 (20 mg/kg daily). In parallel, dimethyl sulfoxide was injected as Vehicle. N = 4–7 mice. Data represent mean ± SEM. *P < 0.05, ****P < 0.0001; Student’s t-test