Fig. 9
Presence of microglia and astrocytes in the SC of 6-month-old TAU58 mice brain after subretinal injection of brain lysates. a: Significant differences were observed in the percentage of GFAP-positive area in the contralateral SC between PBS- and AD-brain lysate-injected mice. However, no significant difference was found between PBS- and non-AD/PART-injected mice (One-way ANOVA with Dunnett’s test, PBS vs AD: p = 0,0426, PBS vs non-AD/PART: p = 0,6175), b: No significant differences were observed in the percentage of the percentage of Iba1-positive area between the examined groups (One-way ANOVA with Dunnett’s test: p > 0,05). Representative merged fluorescence images of the contralateral SC (c-e) and their higher magnifications (f-h) show astrocytes (GFAP) in green, microglia (Iba1) in magenta, pTau (pTauS202/T205) in red, and nuclei (Hoechst) in blue. The white-outlined rectangular area in panel h highlights pTauS202/T205 immunoreactivity localized in proximity to reactive microglia and astrocytes. Single-channel images for the merged images in c-h are shown in Fig. S10; a-d’. Magnifications of panels f, g, and h are derived from panels c, d, and e, respectively. Error bars: Min to Max in a, b. Scale bar in c is valid for panels c-e and scale bar in f is valid for panels f-h. (PBS; n = 10, non-AD/PART; n = 10, AD; n = 10 cases)