Fig. 6

The pTau pathology in the SC of 6-month-old TAU58 mice brain after subretinal injection of brain lysates. a: Schematic representation of a coronal section showing the delineated layers of SC, with superficial layers marked in purple and intermediate and deeper layers (DLSC) marked in blue. The Kruskal-Wallis test, followed by Dunn’s test, was conducted within the layers of the SC in the left (b, d, f, h, j, l, n) and right (c, e, g, i, k, m, o) hemispheres. b: Significant differences in the number of pTau-positive neurons were observed in the zonal layer of the SC between the PBS and AD brain homogenate injected groups (p = 0.0198). j: Similarly, significant differences were noted in the number of pTau-positive neurons in the intermediate white layer of the SC between the PBS and AD brain homogenate injected groups (p = 0.0198). No significant differences were found in the number of pTau-positive neurons between the seed injection groups for panels c, d, e, f, g, h, i, k, l, m, n and o. Panels p-r show fluorescence staining images of the contralateral (left) superior colliculus with zonal layers marked in white after subretinal injection into the right eye of 6-month-old female TAU58 mice for AD, non-AD/PART, and PBS-injected groups. The arrowhead indicates pTau pathology. Panel s: Magnified region from (a), showing neurons positive for pTau (white) in the zonal layer following the injection of AD brain homogenates. The merged image on the left displays neurons (NeuN) in red and nuclei (Hoechst) in blue, with arrowheads indicating pTau^S202/T205 cytoplasmic inclusions. The schematic figure in a was made with the help of the Allen Institute for Brain Science. (2011), pp. http://mouse.brain-map.org. Error bars: Min to Max. SC = superior colliculus, zo = zonal layer, sg = superficial gray layer, op = optic layer, ig = intermediate gray layer, iw = intermediate white layer, dg = deep gray layer, dw = deep white layer